人肝癌细胞HuH-7

细胞名称

人肝癌细胞HuH-7

货物编码

BFN60807349

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研           1类

培养体系

DMEM高糖培养基+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人肝癌细胞HuH-7取自57岁男性供体，该细胞源于ATCC

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 3.

Part of: JFCR45 cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: TCGA-110-CL cell line panel.

Doubling time: ~48 hours (CLS).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Homozygous for KDR p.Gln472His (c.1416A>T) (PubMed=29774518).

POLD3 p.Lys70Arg (c.209A>G) (DOI=10.1101/2020.02.17.953281).

Homozygous for TP53 p.Tyr220Cys (c.659A>G) (PubMed=8389256; PubMed=29774518).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        11

D1S1656        16

D2S1338        19

D3S1358        15

D5S818        12

D6S1043        13,15

D7S820        11

D8S1179        14,15

D12S391        20 (CLS)

20,21 (CCRID)

D13S317        10 (CLS; RCB)

10,11 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; TKG)

D16S539        10

D18S51        15

D19S433        13,14

D21S11        30

FGA        22,23

Penta D        12

Penta E        11

TH01        7

TPOX        8,11

vWA        16,18 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; RCB; TKG)

18 (CLS)

参考文献

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

DOI=10.1101/2020.02.17.953281

Kawamoto M., Yamaji T., Saito K., Satomura K., Endo T., Fukasawa M., Hanada K., Osada N.

Identification of characteristic genomic markers in human hepatoma Huh7 and Huh7.5.1-8 cell lines.

bioRxiv 2020:953281-953281(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)