人肺癌细胞A549

细胞名称

人肺癌细胞A549                  

货物编码

BFN60800665

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途                 1类

培养体系

DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）

培养温度

37℃

二氧化碳浓度

5%

简介

人肺癌细胞A549细胞系是1972年由GiardDJ通过肺癌组织移植培养建系的，源自一位58岁的白人男性。A549能通过胞苷二磷脂酰胆碱途径合成富含不饱和脂肪酸的卵磷脂；角蛋白阳性。 

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 2.

Part of: JFCR39 cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Part of: NCI-60 cancer cell line panel.

Part of: NCI-7 clinical proteomics reference material cell line panel.

Doubling time: 18 hours (in RPMI 1640 + 10% FBS), 37 hours (in ACL-3), 36 hours (in ACL-3+BSA) (PubMed=3940644); 27.0 hours (PubMed=8286010); 22 hours (PubMed=25984343); 27 hours (from cell counting), 27 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); 22.9 hours (NCI-DTP); ~28 hours (CLS); ~40 hours (DSMZ).

Microsatellite instability: Stable (MSS) (PubMed=12661003; Sanger).

Omics: Acetylation analysis by proteomics.

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep membrane proteome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Fluorescence phenotype profiling.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: lncRNA expression profiling.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Proteome analysis by 2D-DE/MS.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Omics: Virome analysis using proteomics.

Misspelling: A594; In PubMed=18227028.

Misspelling: A59; In PubMed=16354588.

STR信息

Amelogenin：X，Y；CSF1PO：10，12；D13S317：11；D16S539：11，12；D18S51：14，17；D19S433：13；D21S11：29；D2S1338：24；D3S1358：16；D5S818：11；D7S820：8，11；D8S1179：13，14；FGA：23；TH01：8，9.3；TPOX：8，11；vWA：14；

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