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BFB

 

人肝癌细胞HuH-7

 

BLUEFBIO Product Sheet

 

细胞名称

人肝癌细HuH-7                  

img1

货物编码

BFN60800691

产品规格

T25培养x1

1.5ml冻存x2

细胞数量

1x10^6

1x10^6

保存温度

37

-198

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途                1类

 

培养体系

DMEM高糖培养基Hyclone+10%胎牛血清Gibco+1%双抗Hyclone

培养温度

37

二氧化碳浓度

5%

简介

人肝癌细HuH-7细胞产甲胎蛋白,胰α抗体,血浆铜蓝蛋白,纤维蛋白原,纤维粘连蛋白等

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 3.

Part of: JFCR45 cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: TCGA-110-CL cell line panel.

Doubling time: ~48 hours (CLS).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

AmelogeninX,XCSF1PO11,11D12S39120,21D13S31710,11D16S53910,10D18S5115,15D19S43313,14D21S1130,30D2S133819,19D3S135815,15D5S81812,12D6S104313,15D7S82011,11D8S117914,15FGA22,22Penta E11,11TH017,7TPOX8,11vWA16,18

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验收细胞注意事 

1、收到人肝癌细HuH-7细胞,请查看瓶子是否有破裂,培养基是否漏出,是否浑浊,如有请尽快联系 

2、收到人肝癌细HuH-7细胞,如包装完好,请在显微镜下观察细胞,由于运输过程中的问题,细胞培养瓶中的贴壁细胞有可能从瓶壁中脱落下来,显微镜下观察会出现细胞悬浮的情况,出现此状态时,请不要打开细胞培养瓶,应立即将培养瓶置于细胞培养箱里静 3-5 小时左右,让细胞先稳定下,再于显微镜下观察,此时多数细胞会重新贴附于瓶壁。如细胞仍不能贴壁,请用台盼蓝染色法鉴定细胞活力,如台盼蓝染色证实细胞活力正常请按悬浮细胞的方法处理 

3、收到人肝癌细HuH-7细胞后,请镜下观察细胞,用恰当方式处理细胞。若悬浮的细胞较多,请离心收集细胞,接种到一个新的培养瓶中。弃掉原液,使用新鲜配制的培养基,使用进口胎牛血清。刚接到细胞,若细胞不多 血清浓度可以加 15%去培养。若细胞迏 80% ,血清浓度还是 10 

4、收到人肝癌细HuH-7细胞时如无异常情 ,请在显微镜下观察细胞密度,如为贴壁细胞,未超80%汇合度时,将培养瓶中培养基吸出,留 5-10ML 培养基继续培养:超 80%汇合度时,请按细胞培养条件传代培养。如为悬浮细胞,吸出培养液1000 /分钟离 3 分钟,吸出上清,管底细胞用新鲜培养基悬浮细胞后移回培养瓶 

5、将培养瓶置 37培养箱中培养,盖子微微拧松。吸出的培养基可以保存在灭菌过的瓶子里,存放 4冰箱,以备不时之需 

624 小时后,人肝癌细HuH-7细胞形态已恢复并贴满瓶壁,即可传代。(贴壁细胞)将培养瓶里的培养基倒去, 3-5ml(以能覆盖细胞生长面为准PBS  Hanks液洗涤后弃去。 0.5-1ml 0.25% EDTA 的胰酶消化,消化时间以具体细胞为准,一 1-3 分钟,不超 5 分钟。可以放37培养箱消化。轻轻晃动瓶壁,见细胞脱落下来,加 3-5ml 培养基终止消化。用移液管轻轻吹打瓶壁上的细胞,使之完全脱落,然后将溶液吸入离心管内离心1000rpm/5min。弃上清,视细胞数量决定分瓶数,一般一传二,如细胞量多可一传三,有些细胞不易传得过稀,有些生长较快的细胞则可以多传几瓶,以具体细胞和经验为准。(悬浮细胞)用移液管轻轻吹打瓶壁,直接将溶液吸入离心管离心即可 

7、贴壁细 ,悬浮细胞。严格无菌操作。换液时,换新的细胞培养瓶和换新鲜的培养液375%CO2 培养。

 

特别提醒 原瓶中培养基不宜继续使用,请更换新鲜培养基培养。