人脐静脉内皮细胞 HUVEC
BLUEFBIO™ Product Sheet
细胞名称 |
人脐静脉内皮细胞 HUVEC |
|
|
货物编码 |
BFN607200285 |
||
产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
|
细胞数量 |
1x10^6 |
1x10^6 |
|
保存温度 |
37℃ |
-198℃ |
|
运输方式 |
常温保温运输 |
干冰运输 |
|
安全等级 |
1 |
||
用途限制 |
仅供科研用途 2类 |
培养体系 |
HUVEC专用培养基 | ||
培养温度 |
37℃ |
二氧化碳浓度 |
5% |
简介 |
人脐静脉内皮细胞HUVEC 源于ATCC(CRL-1730).部分文献称该细胞为HUVEC-C。 |
||
注释 |
Characteristics: Life expectancy of 50 to 60 population doublings (ATCC). 54 PDL (PubMed=28755030). Characteristics: Contains an integrated copy of HHV-6B (CI-HHV-6B) at the distal end of chromosome 9 (PubMed=28755030). Doubling time: ~23.5 hours (between passages 18 and 30), ~67 hours (between passages 24 and 27), ~84 hours (between passages 27 and 30), ~100 hours (between passages 32 and 34) (PubMed=28755030). |
||
STR信息 |
/ |
||
参考文献 |
PubMed=6333682; DOI=10.1073/pnas.81.20.6413 Hoshi H., McKeehan W.L. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. U.S.A. 81:6413-6417(1984)
PubMed=28755030; DOI=10.1007/s10616-017-0119-y Shioda S., Kasai F., Ozawa M., Hirayama N., Satoh M., Kameoka Y., Watanabe K., Shimizu N., Tang H., Mori Y., Kohara A. The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture. Cytotechnology 70:141-152(2018)
PubMed=29892436; DOI=10.1098/rsos.172472 Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A. Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection. R. Soc. Open Sci. 5:172472-172472(2018) |