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BFB

 

上海细胞库-ATCC细胞库-DSMZ细胞库-细胞库    科研细胞2类    人脐静脉内皮细胞 HUVEC

人脐静脉内皮细胞 HUVEC

 

BLUEFBIO Product Sheet

 

细胞名称

人脐静脉内皮细 HUVEC

img1

货物编码

BFN607200285

产品规格

T25培养x1

1.5ml冻存x2

细胞数量

1x10^6

1x10^6

保存温度

37

-198

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途                2类

 

培养体系

HUVEC专用培养基

培养温度

37

二氧化碳浓度

5%

简介

人脐静脉内皮细胞HUVEC 源于ATCCCRL-1730).部分文献称该细胞为HUVEC-C。

注释

Characteristics: Life expectancy of 50 to 60 population doublings (ATCC). 54 PDL (PubMed=28755030).

Characteristics: Contains an integrated copy of HHV-6B (CI-HHV-6B) at the distal end of chromosome 9 (PubMed=28755030).

Doubling time: ~23.5 hours (between passages 18 and 30), ~67 hours (between passages 24 and 27), ~84 hours (between passages 27 and 30), ~100 hours (between passages 32 and 34) (PubMed=28755030).

STR信息

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参考文献

PubMed=6333682; DOI=10.1073/pnas.81.20.6413

Hoshi H., McKeehan W.L.

Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture.

Proc. Natl. Acad. Sci. U.S.A. 81:6413-6417(1984)

 

PubMed=28755030; DOI=10.1007/s10616-017-0119-y

Shioda S., Kasai F., Ozawa M., Hirayama N., Satoh M., Kameoka Y., Watanabe K., Shimizu N., Tang H., Mori Y., Kohara A.

The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

Cytotechnology 70:141-152(2018)

 

PubMed=29892436; DOI=10.1098/rsos.172472

Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.

Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.

R. Soc. Open Sci. 5:172472-172472(2018)