人横纹肌肉瘤细胞RD

细胞名称

人横纹肌肉瘤细胞RD

货物编码

BFN60807544

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研        1类

培养体系

90%DMEM+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人横纹肌肉瘤细胞RD取自7岁女性供体。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.

Doubling time: 23 hours (PubMed=20922763).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Cell surface proteome.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Exosome proteome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Homozygous for NRAS p.Gln61His (c.183A>T) (PubMed=3158613; PubMed=12068308; PubMed=22142829; ATCC).

Homozygous for TP53 p.Arg248Trp (c.742C>T) (PubMed=2216456; ATCC).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,11

D2S1338        17,23

D3S1358        15,17

D5S818        11

D7S820        8,12

D8S1179        11,15

D13S317        13

D16S539        10,11

D18S51        13,18

D19S433        11,14

D21S11        28,29

FGA        20,21

Penta D        11,13

Penta E        12

TH01        9.3

TPOX        9

vWA        18

参考文献

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31448612; DOI=10.1021/acs.jproteome.9b00157

Rammal G., Fahs A., Kobeissy F., Mechref Y., Zhao J., Zhu R., Diab-Assaf M., Saab R., Ghayad S.E.

Proteomic profiling of rhabdomyosarcoma-derived exosomes yield insights into their functional role in paracrine signaling.

J. Proteome Res. 18:3567-3579(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)