人多发性骨髓瘤细胞KHM-1B

细胞名称

人多发性骨髓瘤细胞KHM-1B

货物编码

BFN60807656

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研        2类

培养体系

90%DMEM+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人多发性骨髓瘤细胞KHM-1B取自53岁男性供体。该细胞源于JCRB细胞库。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Characteristics: Produces IgA lambda.

Doubling time: ~2 days (PubMed=2458153).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Pleural effusion.

基因突变

Heterozygous for KRAS p.Gly12Cys (c.34G>T) (CCLE).

NRAS p.Gly12Cys (c.34G>T) (CCLE).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10

D3S1358        15

D5S818        11

D7S820        10

D8S1179        11,12

D13S317        11

D16S539        9

D18S51        16,18

D21S11        30,31

FGA        24

Penta D        9,12

Penta E        8,16

TH01        6

TPOX        11

vWA        14,17

参考文献

PubMed=30285677; DOI=10.1186/s12885-018-4840-5

Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

BMC Cancer 18:940-940(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)