人膀胱移形癌细胞RT112

细胞名称

人膀胱移形癌细胞RT112

货物编码

BFN60808321

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研        3类

培养体系

90%DMEM+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人膀胱移形癌细胞RT112取自女性供体，该细胞源于DSMZ

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: TCGA-110-CL cell line panel.

Part of: UBC-40 urothelial bladder cancer cell line index.

Doubling time: 24 hours (PubMed=3708594); 25 hours (PubMed=25984343); 25.7 hours (PubMed=26055179); ~35 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

FGFR3-TACC3 gene fusion (PubMed=25485619; PubMed=28855393).

TERT c.228C>T (-124C>T); in promoter (PubMed=24035680; PubMed=31068700).

Heterozygous for TP53 p.Ser183Ter (c.548C>G) and p.Arg248Gln (c.743G>A) (CCLE; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,11 (CLS; Cosmic-CLP; DSMZ; PubMed=27270441)

10 (PubMed=25877200)

D3S1358        15

D5S818        10,13

D7S820        11,12

D8S1179        13,15

D13S317        13,14

D16S539        11,13

D18S51        15 (CLS)

15,17 (PubMed=25877200)

D21S11        27,30

FGA        23 (CLS)

23,25 (PubMed=25877200)

Penta D        10,11 (CLS)

10 (PubMed=25877200)

Penta E        12,16

TH01        7

TPOX        8,11

vWA        14,17

参考文献

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)