人乳腺癌细胞HCC1937

细胞名称

人乳腺癌细胞HCC1937

货物编码

BFN60808356

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研        1类

培养体系

90%DMEM+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人乳腺癌细胞HCC1937取自24岁女性。

注释

Group: Triple negative breast cancer (TNBC) cell line.

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: GrayJW Breast Cancer Cell Line Panel.

Part of: ICBP43 breast cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Doubling time: ~50 hours (DSMZ); ~61 hours (PBCF); 53.49 hours (https://www.synapse.org/#!Synapse:syn2347014).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Glycoproteome analysis by proteomics.

Omics: HLA class I peptidome analysis by proteomics.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Homozygous for BRCA1 p.Gln1756Profs*74 (c.5266dupC) (5382insC). The lymphoblastoid cell line HCC1937 BL (CVCL_3281) is heterozygous for the same mutation (PubMed=9699648; PubMed=16397213; PubMed=22032724).

Homozygous for PTEN deletion (PubMed=19593635).

Homozygous for RB1 p.Thr738_Arg775del38 (c.2212_2325del114) (PubMed=19593635).

HLA信息

Class I

HLA-A        A*23:01,24:02

HLA-B        B*40:01,07:02

HLA-C        C*07:02,03:04

Class II

HLA-DQ        DQA1*04:01,04:01

DQB1*06:02,06:02

HLA-DR        DRB1*15:01,08:01

STR信息

Amelogenin        X

CSF1PO        12

D3S1358        18

D5S818        12

D7S820        9,10

D8S1179        12,13

D13S317        13

D16S539        13,14

D18S51        12

D21S11        28

FGA        20,22

Penta D        9

Penta E        13

TH01        6,8 (KCLB)

6 (ATCC; Cosmic-CLP; DSMZ; PubMed=25877200; PubMed=28889351)

TPOX        11

vWA        16,17

参考文献

PubMed=28889351; DOI=10.1007/s10549-017-4496-x

Saunus J.M., Smart C.E., Kutasovic J.R., Johnston R.L., Kalita-de Croft P., Miranda M., Rozali E.N., Vargas A.C., Reid L.E., Lorsy E., Cocciardi S., Seidens T., McCart Reed A.E., Dalley A.J., Wockner L.F., Johnson J., Sarkar D., Askarian-Amiri M.E., Simpson P.T., Khanna K.K., Chenevix-Trench G., Al-Ejeh F., Lakhani S.R.

Multidimensional phenotyping of breast cancer cell lines to guide preclinical research.

Breast Cancer Res. Treat. 167:289-301(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)