人胃癌细胞NCI-N87

细胞名称

人胃癌细胞NCI-N87

货物编码

BFN60808579

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研      2类

培养体系

DMEM高糖培养基+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人胃癌细胞NCI-N87取自男性供体，年龄未知。该细胞源于ATCC

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MET genetic alteration cell panel (ATCC TCP-1036).

Doubling time: 47 hours (PubMed=2158397); 86 hours (PubMed=25984343); 23 hours (PubMed=26919099).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Misspelling: NU-N87; In PubMed=25960936 and NCBI SRX181255.

基因突变

ERBB3 p.Thr757Lys (c.2270C>A) (PubMed=26919099).

Homozygous for SMAD4 deletion (ATCC).

Homozygous for TP53 p.Arg248Gln (c.743G>A) (PubMed=1676761; ATCC).

HLA信息

Class I

HLA-A        A*23:01,01:01

HLA-B        B*37:01,07:02

HLA-C        C*02:02,07:02

Class II

HLA-DQ        DQB1*05:02,05:02

HLA-DR        DRB1*15:01,15:01

STR信息

Amelogenin        X,Y

CSF1PO        8,12

D2S441        11,12

D2S1338        23,24

D3S1358        14

D5S818        12,13

D6S1043        12

D7S820        10,11

D8S1179        14,15

D12S391        16,21

D13S317        8,11

D16S539        9,13

D18S51        17

D19S433        14,14.2

D21S11        30

FGA        20,21

Penta D        12

Penta E        5

TH01        7,9 (PubMed=27102572)

9 (AddexBio; ATCC; CCRID; Cosmic-CLP; PubMed=25877200)

TPOX        9,11

vWA        15,16

参考文献

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)