人间皮细胞LP9

细胞名称

人间皮细胞LP9

货物编码

BFN60808632

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研      2类

培养体系

DMEM高糖+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人间皮细胞LP9取自26岁女性供体，腹水来源。贴壁培养。

注释

Characteristics: Senesces at ~50-60 PDL (PubMed=6192933; PubMed=10648628).

Derived from sampling site: Ascites.

基因突变

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HLA信息

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STR信息

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参考文献

PubMed=6186388; DOI=10.1016/0092-8674(82)90324-5

Wu Y.-J., Parker L.M., Binder N.E., Beckett M.A., Sinard J.H., Griffiths C.T., Rheinwald J.G.

The mesothelial keratins: a new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia.

Cell 31:693-703(1982)

PubMed=6192933; DOI=10.1016/0092-8674(83)90155-1

Connell N.D., Rheinwald J.G.

Regulation of the cytoskeleton in mesothelial cells: reversible loss of keratin and increase in vimentin during rapid growth in culture.

Cell 34:245-253(1983)

PubMed=3130603

Tubo R.A., Rheinwald J.G.

Normal human mesothelial cells and fibroblasts transfected with the EJras oncogene become EGF-independent, but are not malignantly transformed.

Oncogene Res. 1:407-421(1987)

PubMed=9382953; DOI=10.1089/hum.1997.8.16-1867

Murphy J.-E., Rheinwald J.G.

Intraperitoneal injection of genetically modified, human mesothelial cells for systemic gene therapy.

Hum. Gene Ther. 8:1867-1879(1997)

PubMed=10648628; DOI=10.1128/MCB.20.4.1436-1447.2000

Dickson M.A., Hahn W.C., Ino Y., Ronfard V., Wu J.Y., Weinberg R.A., Louis D.N., Li F.P., Rheinwald J.G.

Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics.

Mol. Cell. Biol. 20:1436-1447(2000)

PubMed=19489101; DOI=10.1002/stem.64

Dabelsteen S., Hercule P., Barron P., Rice M., Dorsainville G., Rheinwald J.G.

Epithelial cells derived from human embryonic stem cells display p16INK4A senescence, hypermotility, and differentiation properties shared by many P63+ somatic cell types.

Stem Cells 27:1388-1399(2009)