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上海细胞库-ATCC细胞库-DSMZ细胞库-细胞库    科研细胞2类    人脐静脉内皮细胞融合细胞EA.hy926

人脐静脉内皮细胞融合细胞EA.hy926

 

BLUEFBIO Product Sheet

 

细胞名称

人脐静脉内皮细胞融合细EA.hy926

img1

货物编码

BFN60808855

产品规格

T25培养x1

1.5ml冻存x2

细胞数量

1x10^6

1x10^6

保存温度

37

-198

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科   2

 

培养体系

内皮细胞专用培养基

培养温度

37

二氧化碳浓度

5%

简介

人脐静脉内皮细胞融合细EA.hy926,该细胞为脐静脉内皮细胞A549/8的融合细胞。该细胞保藏ATCC

注释

Group: Space-flown cell line (cellonaut).

Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells.

Doubling time: 12 hours (PubMed=6407019).

Omics: Transcriptome analysis.

Anecdotal: Have been flown in space on Shijian-10 and on the ISS in 2016 to study growth and secretion in microgravity (PubMed=28199884; PubMed=30108515; PubMed=30977987).

Misspelling: EA.hy 296; Occasionally.

Misspelling: EA-hy 296; Occasionally.

Misspelling: EA.hy296; Occasionally.

基因突变

Homozygous for KRAS p.Gly12Ser (c.34G>A) (ClinVar=VCV000012584) (from parent cell line).

Homozygous for STK11 p.Gln37Ter (c.109C>T) (ClinVar=VCV000376334) (from parent cell line).

HLA信息

 

STR信息

Amelogenin        X

CSF1PO        10,11,12

D5S818        11

D7S820        8,9,10

D13S317        11

D16S539        11,12

TH01        6,8,9.3

TPOX        8,9

vWA        14,17

参考文献

PubMed=16575254; DOI=10.1097/01.mbc.0000220237.99843.a1

Boerma M., Burton G.R., Wang J., Fink L.M., McGehee R.E. Jr., Hauer-Jensen M.

Comparative expression profiling in primary and immortalized endothelial cells: changes in gene expression in response to hydroxy methylglutaryl-coenzyme A reductase inhibition.

Blood Coagul. Fibrinolysis 17:173-180(2006)

 

PubMed=28199884; DOI=10.1016/j.biomaterials.2017.02.005

Pietsch J., Gass S., Nebuloni S., Echegoyen D., Riwaldt S., Baake C., Bauer J., Corydon T.J., Egli M., Infanger M., Grimm D.

Three-dimensional growth of human endothelial cells in an automated cell culture experiment container during the SpaceX CRS-8 ISS space mission -- The SPHEROIDS project.

Biomaterials 124:126-156(2017)

 

PubMed=30108515; DOI=10.3389/fphys.2018.01025

Li N., Wang C.-Z., Sun S.-J., Zhang C., Lu D.-Y., Chen Q., Long M.

Microgravity-induced alterations of inflammation-related mechanotransduction in endothelial cells on board SJ-10 satellite.

Front. Physiol. 9:1025-1025(2018)

 

PubMed=30977987; DOI=10.33594/000000071

Kruger M., Pietsch J., Bauer J., Kopp S., Carvalho D.T.O., Baatout S., Moreels M., Melnik D., Wehland M., Egli M., Jayashree S., Kobbero S.D., Corydon T.J., Nebuloni S., Gass S., Evert M., Infanger M., Grimm D.

Growth of endothelial cells in space and in simulated microgravity -- a comparison on the secretory level.

Cell. Physiol. Biochem. 52:1039-1060(2019)