猫肾细胞CRFK

细胞名称

猫肾细胞CRFK

货物编码

BFN60810344

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

90%DMEM+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

猫肾细胞CRFK取自雌性个体，贴壁培养。该细胞保藏于ATCC

注释

Group: Vaccine production cell line.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Biotechnology: Widely used to manufacture vaccines for parvoviruses such as feline panleukopenia virus (FPLV) and canine parvoviruses (CPVs).

Characteristics: Persistently infected by feline endogenous virus RD-114.

基因突变

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HLA信息

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STR信息

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参考文献

PubMed=9696876

Baumann J.G., Gunzburg W.H., Salmons B.

CrFK feline kidney cells produce an RD114-like endogenous virus that can package murine leukemia virus-based vectors.

J. Virol. 72:7685-7687(1998)

PubMed=20631117; DOI=10.1128/JCM.00992-10

Yoshikawa R., Sato E., Igarashi T., Miyazawa T.

Characterization of RD-114 virus isolated from a commercial canine vaccine manufactured using CRFK cells.

J. Clin. Microbiol. 48:3366-3369(2010)

PubMed=21029758; DOI=10.1016/j.virusres.2010.10.020

Okada M., Yoshikawa R., Shojima T., Baba K., Miyazawa T.

Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines.

Virus Res. 155:268-273(2011)

PubMed=23585909; DOI=10.1371/journal.pone.0061530

Fukuma A., Yoshikawa R., Miyazawa T., Yasuda J.

A new approach to establish a cell line with reduced risk of endogenous retroviruses.

PLoS ONE 8:E61530-E61530(2013)