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BFB

 

人B淋巴细胞GM12878

 

BLUEFBIO Product Sheet

 

细胞名称

B淋巴细GM12878

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货物编码

BFN60810358

产品规格

T25培养x1

1.5ml冻存x2

细胞数量

1x10^6

1x10^6

保存温度

37

-198

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科   3

 

培养体系

90%DMEM(不含丙酮酸钠+10%FBS+1%三抗

培养温度

37

二氧化碳浓度

5%

简介

B淋巴细GM12878取自女性外周血。

注释

Part of: CEPH/Utah pedigree cell line collection.

Part of: ENCODE project common cell types; tier 1.

Part of: Genetic Testing Reference Material (GeT-RM) samples.

Part of: Genome in a Bottle (GIAB) consortium samples.

Part of: International Genome Sample Resource (1000 genomes project) cell lines.

Registration: CEPH Families Reference Panel; 146302.

Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001.

Population: Utah residents with ancestry from Northern and Western Europe.

Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: DNA methylation analysis.

Omics: Deep RNAseq analysis.

Omics: Genome sequenced.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K4me2 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: H3K9me3 ChIP-seq epigenome analysis.

Omics: H4K20me1 ChIP-seq epigenome analysis.

Omics: Mitochondrial genome sequenced.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突变

CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C19*2 (Coriell).

HLA信息

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STR信息

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参考文献

PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25

Morcos L., Ge B., Koka V., Lam K.C., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T.

Genome-wide assessment of imprinted expression in human cells.

Genome Biol. 12:R25.1-R25.14(2011)

 

PubMed=23325432; DOI=10.1101/gr.147942.112

Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.

Dynamic DNA methylation across diverse human cell lines and tissues.

Genome Res. 23:555-567(2013)

 

PubMed=23676674; DOI=10.1038/nature12223

Wu L., Candille S.I., Choi Y., Xie D., Jiang L., Li-Pook-Than J., Tang H., Snyder M.

Variation and genetic control of protein abundance in humans.

Nature 499:79-82(2013)

 

PubMed=24185094; DOI=10.1038/nbt.2728

Selvaraj S., Dixon J.R., Bansal V., Ren B.

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

Nat. Biotechnol. 31:1111-1118(2013)

 

PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005

Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.H., Toji L.H., Turner A., Kalman L.V.

Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project.

J. Mol. Diagn. 18:109-123(2016)

 

PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096

Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B.

Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines.

Cell Rep. 25:2617-2633(2018)

 

PubMed=31273215; DOI=10.1038/s41597-019-0116-4

Osorio D., Yu X., Yu P., Serpedin E., Cai J.J.

Single-cell RNA sequencing of a European and an African lymphoblastoid cell line.

Sci. Data 6:112-112(2019)

 

PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007

Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V.

Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project.

J. Mol. Diagn. 21:1034-1052(2019)