人急性髓细胞白血病细胞PL21

细胞名称

人急性髓细胞白血病细胞PL21

货物编码

BFN60810477

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

90%DMEM(不含丙酮酸钠）+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人急性髓细胞白血病细胞PL21取自24岁男性外周血。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Doubling time: 48-64 hours (PubMed=6362749); ~40-50 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突变

FLT3 internal tandem duplication (FLT3-ITD) (PubMed=12529668).

Heterozygous for KRAS p.Ala176Val (c.437C>T) (CCLE; Cosmic-CLP).

Heterozygous for TP53 p.Pro36fs*8 (c.107delC) (Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X,Y

CSF1PO        11,12

D3S1358        15

D5S818        10

D7S820        12,13

D8S1179        10

D13S317        8,11

D16S539        11,13

D18S51        12,13

D21S11        29,30

FGA        21,24

Penta D        11,13

Penta E        5,17

TH01        9

TPOX        9,11

vWA        14,18

参考文献

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Cancer Res. 79:1263-1273(2019)

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Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)