人结肠腺癌细胞SW403

细胞名称

人结肠腺癌细胞SW403

货物编码

BFN60810503

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

90%DMEM(不含丙酮酸钠）+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人结肠腺癌细胞SW403取自51岁女性供体。该细胞源于DSMZ

注释

Part of: AstraZeneca Colorectal cell line (AZCL) panel.

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: KuDOS 95 cell line panel.

Part of: NCI RAS program mutant KRAS cell line panel.

From: Scott and White Clinic; Temple; USA.

Doubling time: 79 hours, at 31th passage (PubMed=1000501); ~40-50 hours (DSMZ).

Microsatellite instability: Stable (MSS) (PubMed=25926053; PubMed=28683746; Sanger).

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

APC p.Asn125Lys (c.375T>G), p.Ser1198fs*67 (c.3591delC) and p.Ser1198Leu (c.3593C>T) (not confirmed by Sanger sequencing) (PubMed=24755471).

Heterozygous for KRAS p.Gly12Val (c.35G>T) (ClinVar=VCV000012583) (PubMed=12068308; PubMed=20570890; PubMed=24755471; PubMed=28683746).

PIK3CA p.Gln546Lys (c.1636C>A) (PubMed=24755471).

TP53 p.Glu51Ter (c.151G>T) (PubMed=16418264; PubMed=28683746).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,13

D3S1358        15

D5S818        11

D7S820        8,9

D8S1179        11 (CLS)

11,14 (PubMed=25877200)

D13S317        13

D16S539        10,12

D18S51        17

D21S11        28,29

FGA        19

Penta D        9

Penta E        5

TH01        6

TPOX        8,9

vWA        14,18

参考文献

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402.e16(2020)