人乳腺癌细胞MDA-MB-157

细胞名称

人乳腺癌细胞MDA-MB-157                 

货物编码

BFN60700177

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途             1类

培养体系

DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）

培养温度

37℃

二氧化碳浓度

5%

简介

人乳腺癌细胞MDA-MB-157细胞源自一位患有乳腺髓样癌的44岁黑人女性，表达WNT7B癌基因，细胞与细胞边界处有细胞桥粒、微绒毛、张力细丝。该细胞引种自ATCC HTB-24

注释

Group: Triple negative breast cancer (TNBC) cell line.

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: GrayJW Breast Cancer Cell Line Panel.

Part of: ICBP43 breast cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Doubling time: 2.8 days (PubMed=9671407); 66.98 hours (https://www.synapse.org/#!Synapse:syn2347014).

Microsatellite instability: Stable (MSS) (PubMed=15677628; Sanger).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Glycoproteome analysis by proteomics.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Misspelling: MDA-MB-15; In Cosmic 897421.

STR信息

Amelogenin        X

CSF1PO        10

D2S1338        25

D3S1358        16

D5S818        12

D7S820        10,11

D8S1179        12,14

D13S317        11 (AddexBio; CCRID; Cosmic-CLP; ECACC; PubMed=20679594; PubMed=25877200; PubMed=28889351)

11,12 (ATCC)

D16S539        11

D18S51        16

D19S433        14.2

D21S11        31,31.2

FGA        23

Penta D        3.2,9

Penta E        11

TH01        7,8

TPOX        9,11 (AddexBio; ATCC; ECACC; PubMed=20679594; PubMed=25877200; PubMed=28889351)

11 (CCRID; Cosmic-CLP)

vWA        15

参考文献

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)