人胰腺癌细胞PaTu 8902

细胞名称

人胰腺癌细胞PaTu 8902

货物编码

BFN60870146

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人胰腺癌细胞PaTu 8902 取自44岁女性。该细胞保藏于DSMZ。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Doubling time: ~25-40 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Heterozygous for EP300 p.Ser1650Tyr (6148C>A) (PubMed=10700188; CCLE; Cosmic-CLP).

Heterozygous for KRAS p.Gly12Val (c.35G>T) (ClinVar=VCV000012583) (CCLE; Cosmic-CLP).

Homozygous for TP53 p.Cys176Ser (c.526T>A) (ClinVar=VCV000376570) (PubMed=10700188; CCLE; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin X

CSF1PO 10,11

D3S1358 16,18

D5S818 11,12

D7S820 8,10

D8S1179 13

D13S317 11,12

D16S539 9,11

D18S51 15

D21S11 30

FGA 23

Penta D 11

Penta E 12,15

TH01 9.3

TPOX 8

vWA 18

参考文献

PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051

 Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.

 Differential effector engagement by oncogenic KRAS.

 Cell Rep. 22:1889-1902(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

 An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

 Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

 Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

 Next-generation characterization of the Cancer Cell Line Encyclopedia.

 Nature 569:503-508(2019)