人肺癌细胞A427
BLUEFBIO™ Product Sheet
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细胞名称 |
人肺癌细胞A427 |
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货物编码 |
BFN60870154 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研 3类 |
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培养体系 |
DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗 |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
人肺癌细胞A427取自52岁男性，该细胞保藏于ATCC |
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注释 |
Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: MD Anderson Cell Lines Project. Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982). Doubling time: 28 hours (in RPMI 1640 + 10% FBS), 38 hours (in ACL-3), 42 hours (in ACL-3+BSA) (PubMed=3940644); 36.6 hours (PubMed=29681454); ~60-70 hours (DSMZ). Microsatellite instability: Stable (MSS) (Sanger). Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: Protein expression by reverse-phase protein arrays. Omics: SNP array analysis. Omics: Transcriptome analysis. |
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基因突变 |
Homozygous for CTNNB1 p.Thr41Ala (c.121A>G) (ClinVar=VCV000017580) (ATCC; CCLE). Heterozygous for KRAS p.Gly12Asp (c.35G>A) (ClinVar=VCV000012582) (PubMed=1855224; PubMed=3945555; ATCC; CCLE; Cosmic-CLP). |
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HLA信息 |
/ |
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STR信息 |
Amelogenin X,Y CSF1PO 10,12 D3S1358 16 D5S818 12 D7S820 8,12 D8S1179 12,13 D13S317 11,12 D16S539 11,13 D18S51 12 D21S11 31.2 FGA 18 Penta D 13 Penta E 15,17 TH01 9 TPOX 8,11 vWA 17 |
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参考文献 |
Chemistry-first approach for nomination of personalized treatment in lung cancer. Cell 173:864-878.e29(2018)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A. An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines. Cancer Res. 79:1263-1273(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3 Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R. Next-generation characterization of the Cancer Cell Line Encyclopedia. Nature 569:503-508(2019)
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