人急性单核白血病单核细胞AML-193

细胞名称

人急性单核白血病单核细胞AML-193

货物编码

BFN680347

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗+200ug/ml IL2

培养温度

37℃

二氧化碳浓度

5%

简介

人急性单核白血病单核细胞AML-193取自13岁女性，悬浮培养。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Characteristics: CSF2 and IL3 dependent.

Doubling time: 65 hours (PubMed=25984343); ~50-60 hours (DSMZ).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

BCORL1 p.Arg784Ter (c.2350C>T) (PubMed=21989985; CCLE).

Heterozygous for NRAS p.Gly13Val (c.38G>T) (ClinVar=VCV000375876) (PubMed=12068308; CCLE).

TP53 c.993+2T>G (ClinVar=VCV000635384); splice donor mutation (CCLE).

HLA信息

/

STR信息

Amelogenin X

CSF1PO 11,12

D5S818 9,13

D7S820 9,12

D13S317 12

D16S539 12

TH01 7,9

TPOX 8,9

vWA 15,17

参考文献

PubMed=30285677; DOI=10.1186/s12885-018-4840-5

 Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.

 Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

 BMC Cancer 18:940-940(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

 An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

 Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

 Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

 Next-generation characterization of the Cancer Cell Line Encyclopedia.

 Nature 569:503-508(2019)