人视网膜母细胞瘤细胞WERI-Rb-1
BLUEFBIO™ Product Sheet
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细胞名称 |
人视网膜母细胞瘤细胞WERI-Rb-1 |
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货物编码 |
BFN6021607 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研用途 3类 |
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培养体系 |
DMEM高糖培养基（不含丙酮酸钠）+10%FBS+1%三抗 |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
人视网膜母细胞瘤细胞WERI-Rb-1取自女性供体，该细胞引种自JCRB |
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注释 |
Part of: ENCODE project common cell types; tier 3. Doubling time: 96 hours (PubMed=844036); 50 hours (PubMed=25326674); 84 hours (PubMed=31781967); ~49 hours (DSMZ). Omics: H3K4me3 ChIP-seq epigenome analysis. Omics: SNP array analysis. Omics: Transcriptome analysis. |
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突变信息 |
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STR信息 |
Amelogenin X CSF1PO 10 (TKG) 10,12,13 (ATCC; DepMap; DSMZ; RCB) 11,12 (JCRB) D3S1358 14,15 D5S818 11,12 D7S820 10,12,13 (JCRB) 10,13 (ATCC; DepMap; DSMZ; RCB; TKG) D8S1179 12,15 D13S317 14 D16S539 13 D18S51 13,19 D21S11 29 FGA 21 Penta D 12,13 Penta E 7,11 TH01 9.3 TPOX 8,11 vWA 17,18
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参考文献 |
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458 Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A. A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers. Cancer Res. 70:2158-2164(2010)
PubMed=23498719; DOI=10.1016/S1470-2045(13)70045-7 Rushlow D.E., Mol B.M., Kennett J.Y., Yee S., Pajovic S., Theriault B.L., Prigoda-Lee N.L., Spencer C., Dimaras H., Corson T.W., Pang R., Massey C., Godbout R., Jiang Z., Zacksenhaus E., Paton K., Moll A.C., Houdayer C., Raizis A., Halliday W., Lam W.L., Boutros P.C., Lohmann D., Dorsman J.C., Gallie B.L. Characterisation of retinoblastomas without RB1 mutations: genomic, gene expression, and clinical studies. Lancet Oncol. 14:327-334(2013)
PubMed=25326674; DOI=10.1007/s00418-014-1285-z Busch M., Philippeit C., Weise A., Dunker N. Re-characterization of established human retinoblastoma cell lines. Histochem. Cell Biol. 143:325-338(2015)
PubMed=27115612; DOI=10.1371/journal.pone.0153323 Kooi I.E., Mol B.M., Massink M.P., de Jong M.C., de Graaf P., van der Valk P., Meijers-Heijboer H., Kaspers G.J.L., Moll A.C., Te Riele H., Cloos J., Dorsman J.C. A meta-analysis of retinoblastoma copy numbers refines the list of possible driver genes involved in tumor progression. PLoS ONE 11:E0153323-E0153323(2016)
PubMed=28713896; DOI=10.3892/ijmm.2017.3058 Liu Y., Fan Z.-F., Li K., Deng F., Xiong Y.-F., Liang M.-X., Ge J. An optimized gene transfection system in WERI-Rb1 cells. Int. J. Mol. Med. 40:801-813(2017)
PubMed=30584916; DOI=10.1016/j.yexcr.2018.12.018 Schwermer M., Hiber M., Dreesmann S., Rieb A., Theissen J., Herold T., Schramm A., Temming P., Steenpass L. Comprehensive characterization of RB1 mutant and MYCN amplified retinoblastoma cell lines. Exp. Cell Res. 375:92-99(2019)
PubMed=31781967; DOI=10.1007/s00418-019-01832-1 Ravishankar H., Mangani A.S., Shankar M.B., Joshi M., Devasena T., Parameswaran S., Subramaniam K. Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site. Histochem. Cell Biol. 153:101-109(2020)
PubMed=32123578; DOI=10.1038/s41420-020-0237-8 Aubry A., Yu T., Bremner R. Preclinical studies reveal MLN4924 is a promising new retinoblastoma therapy. Cell Death Discov. 6:2-2(2020)
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