人胚肺成纤维细胞Walvax-2

细胞名称

人胚肺成纤维细胞Walvax-2

货物编码

BFN60811422

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

DMEM(HYCLONE SH30022.01)+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人胚肺成纤维细胞Walvax-2取自3月流产（医学原因）女性胎儿，贴壁培养。

注释

Group: Vaccine production cell line.

Registration: International Depositary Authority, China Center for Type Culture Collection; CCTCC C201055.

Characteristics: Maintains excellent capabilities for growth and proliferation until PDL 50 (max PDL is 58).

Doubling time: 39-41 hours (at 28th passage), 40-42 hours (at 38th passage), 57-62 hours (at 48th passage) (PubMed=25803132).

Anecdotal: Is causing an outrage in some pro-lifer circles because it is a cell line originating from an aborted (for medical reasons) 3-month old female fetus.

基因突变

/

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,12

D2S1338        20,25

D3S1358        15,16

D5S818        10,11

D6S1043        14,19

D7S820        8,12

D8S1179        13,15

D12S391        19,22

D13S317        11,12

D16S539        9,12

D18S51        15,18

D19S433        13.2,14.2

D21S11        29,30

FGA        21,24

Penta D        10,11

Penta E        5,18

TH01        6,9

TPOX        8,11

vWA        18

参考文献

Patent=CN102911910A

Huang Z., He L.-F., Zhang W., Ma B., Wang L.-L., Zhao Q.-Y., Zhang Y., Wu W.-J., Chen M., Wang X., Tang Y.-F.

Human embryo lung fibroblast strain and method for using human embryo lung fibroblast strain for producing hand-foot-mouth viral vaccine.

Patent number CN102911910A, 06-Feb-2013

Patent=CN103525770B

Ma B., Yang Z., He L.-F., Wang L.-L., Chen M., Wang X.

Application of human fetal lung fibroblast line in preparation of hepatitis A vaccines.

Patent number CN103525770B, 19-Aug-2015

PubMed=25803132; DOI=10.1080/21645515.2015.1009811

Ma B., He L.-F., Zhang Y.-L., Chen M., Wang L.-L., Yang H.-W., Yan T., Sun M.-X., Zheng C.-Y.

Characteristics and viral propagation properties of a new human diploid cell line, Walvax-2, and its suitability as a candidate cell substrate for vaccine production.

Hum. Vaccin. Immunother. 11:998-1009(2015)

PubMed=26116706; DOI=10.1096.14-266718

Ye F., Chen C.-G., Qin J., Liu J., Zheng C.-Y.

Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China.

FASEB J. 29:4268-4272(2015)