人乳腺上皮细胞MCF-10A
BLUEFBIO™ Product Sheet
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细胞名称 |
人乳腺上皮细胞MCF-10A |
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货物编码 |
BFN60805246 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研 1类 |
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培养体系 |
DMEM高糖+10%FBS+1%双抗 |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
人乳腺上皮细胞MCF-10A取自36岁女性供体。该细胞引种自ATCC CRL-10317 |
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注释 |
Part of: GrayJW Breast Cancer Cell Line Panel. Part of: ICBP43 breast cancer cell line panel. Part of: MD Anderson Cell Lines Project. Doubling time: 26.74 hours (https://www.synapse.org/#!Synapse:syn2347014). Microsatellite instability: Stable (MSS) (PubMed=12661003). Omics: CNV analysis. Omics: Deep proteome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: Glycoproteome analysis by proteomics. Omics: Metabolome analysis. Omics: N-glycan profiling. Omics: Protein expression by reverse-phase protein arrays. Omics: SNP array analysis. Omics: Transcriptome analysis. |
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STR信息 |
Amelogenin X CSF1PO 10,12 D2S1338 21,26 D3S1358 14,18 D5S818 10,13 D6S1043 12,18 D7S820 10,11 D8S1179 14,16 D12S391 17,20 D13S317 8,9 D16S539 11,12 D18S51 18,19 D19S433 13,15 D21S11 28,30 FGA 22,24 Penta D 10,12 Penta E 13,14 TH01 8,9.3 TPOX 9,11 vWA 15,17 |
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参考文献 |
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005 Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H. Characterization of human cancer cell lines by reverse-phase protein arrays. Cancer Cell 31:225-239(2017)
PubMed=28287265; DOI=10.1021/acs.jproteome.6b00470 Yen T.-Y., Bowen S., Yen R., Piryatinska A., Macher B.A., Timpe L.C. Glycoproteins in claudin-low breast cancer cell lines have a unique expression profile. J. Proteome Res. 16:1391-1400(2017)
PubMed=28596718; DOI=10.1007/s11306-017-1213-z Herman S., Emami Khoonsari P., Aftab O., Krishnan S., Strombom E., Larsson R., Hammerling U., Spjuth O., Kultima K., Gustafsson M. Mass spectrometry based metabolomics for in vitro systems pharmacology: pitfalls, challenges, and computational solutions. Metabolomics 13:79-79(2017)
PubMed=28889351; DOI=10.1007/s10549-017-4496-x Saunus J.M., Smart C.E., Kutasovic J.R., Johnston R.L., Kalita-de Croft P., Miranda M., Rozali E.N., Vargas A.C., Reid L.E., Lorsy E., Cocciardi S., Seidens T., McCart Reed A.E., Dalley A.J., Wockner L.F., Johnson J., Sarkar D., Askarian-Amiri M.E., Simpson P.T., Khanna K.K., Chenevix-Trench G., Al-Ejeh F., Lakhani S.R. Multidimensional phenotyping of breast cancer cell lines to guide preclinical research. Breast Cancer Res. Treat. 167:289-301(2018)
PubMed=30787054; DOI=10.1158/1055-9965.EPI-18-1132 Hooker S.E., Woods-Burnham L., Bathina M., Lloyd S.M., Gorjala P., Mitra R., Nonn L., Kimbro K.S., Kittles R. Genetic ancestry analysis reveals misclassification of commonly used cancer cell lines. Cancer Epidemiol. Biomarkers Prev. 28:1003-1009(2019) |
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