人子宫平滑肌肉瘤细胞SK-UT-1

细胞名称

人子宫平滑肌肉瘤细胞SK-UT-1

货物编码

BFN60870118

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   3类

培养体系

DMEM高糖（HYCLONE SH30022.01）+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人子宫平滑肌肉瘤细胞SK-UT-1取自75岁女性供体。该细胞保藏于ATCC

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

From: Memorial Sloan Kettering Cancer Center; New York; USA.

Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-537.

Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Heterozygous for APC p.Gln1096Ter (c.3286C>T) and p.Thr1556fs*9 (c.4666delA) (ATCC).

Heterozygous for PIK3CA p.Arg88Gln (c.263G>A) (ATCC).

Heterozygous for PTEN p.Thr319fs*1 (c.955_958delACTT) (V317fs*3) and p.Asn323fs*2 (c.968_969insA) (ATCC).

Homozygous for RB1 p.Val654fs*4 (c.1959delA) (ATCC).

Heterozygous for TP53 p.Arg175His (c.524G>A) (ClinVar=VCV000012374) and p.Arg248Gln (c.743G>A) (ClinVar=VCV000012356) (PubMed=2216456; ATCC).

HLA信息

/

STR信息

Amelogenin X

CSF1PO 10,11

D3S1358 15,16

D5S818 10,11

D7S820 9,10

D8S1179 13,15

D13S317 13

D16S539 13,14

D18S51 11,16

D21S11 29,32.2

FGA 22,24

Penta D 11,15

Penta E 17

TH01 7

TPOX 8

vWA 15,16

参考文献

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

 Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

 A landscape of pharmacogenomic interactions in cancer.

 Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

 An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

 Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

 Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

 Next-generation characterization of the Cancer Cell Line Encyclopedia.

 Nature 569:503-508(2019)