人非小细胞肺癌细胞NCI-H358

细胞名称

人非小细胞肺癌细胞NCI-H358                  

货物编码

BFN60800666

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途                  1类

培养体系

DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）

培养温度

37℃

二氧化碳浓度

5%

简介

人非小细胞肺癌细胞NCI-H358细胞于1981年从一位开始化疗之前的患者的肿瘤组织中分离建株。超微结构研究表明细胞质中有Clara细胞的特征结构细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。不表达SP-B和SP-C。他们在软琼脂中的克隆形成效率为0.83%。 

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Doubling time: 38 hours (in RPMI 1640 + 10% FBS), 76 hours (in ACL-3), 60 hours (in ACL-3 + BSA) (PubMed=3940644); 38 hours (ECACC).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Caution: We are not certain that NCI-H358 and NCI-H358M are identical.

Misspelling: H1358; In PubMed=1311061 table 1.

STR信息

 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17

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