人肺鳞癌细胞NCI-H520

细胞名称

人肺鳞癌细胞NCI-H520                

货物编码

BFN60700297

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途              1类

培养体系

DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）

培养温度

37℃

二氧化碳浓度

5%

简介

人肺鳞癌细胞NCI-H520于1982年由GazdarAF等建系，源于鳞状细胞肺癌组织；该细胞p53mRNA表达水平较正常肺组织低，但是没有大的结构DNA的异常。角蛋白、波形蛋白阳性，神经丝三联蛋白阴性；该细胞在软琼脂中可形成克隆。引种自ATCC HTB-182

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Doubling time: 61 hours (in RPMI 1640 + 10% FBS), 25 hours (in ACL-3), 32 hours (in ACL-3 + BSA) (PubMed=3940644).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

Amelogenin        X

CSF1PO        10

D3S1358        16

D5S818        12,13 (AddexBio; ATCC; Cosmic-CLP)

12 (PubMed=25877200)

D7S820        8,12

D8S1179        14,16,17

D13S317        10,11

D16S539        8,13

D18S51        17

D21S11        30

FGA        22

Penta D        13

Penta E        5,14

TH01        10

TPOX        8

vWA        18,19

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