人结肠腺癌细胞SW48

细胞名称

人结肠腺癌细胞SW48

货物编码

BFN60808301

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研        1类

培养体系

90%DMEM+10%FBS+1%双抗 

培养温度

37℃

二氧化碳浓度

5%

简介

人结肠腺癌细胞SW48取自85岁女性供体，该细胞源于ATCC

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: EGFR genetic alteration cell panel (ATCC TCP-1027).

Part of: MD Anderson Cell Lines Project.

From: Scott and White Clinic; Temple; USA.

Doubling time: 70 hours, at 63th passage (PubMed=1000501); 33 hours (PubMed=25984343).

Microsatellite instability: Instable (MSI-high) (PubMed=9000572; PubMed=24042735; PubMed=25926053; PubMed=28683746; PubMed=31068700; Sanger).

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative phosphoproteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: N-glycan profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突变

Homozygous for ACVR2A p.Lys437fs*5 (c.1310delA) (PubMed=12615714).

APC p.Arg2714Cys (c.8140C>T) (PubMed=24755471).

Heterozygous for B2M p.Ser14fs*29 (c.41_44delCTCT) and p.Glu67fs (c.200delA) (PubMed=8197130).

Heterozygous for CTNNB1 p.Ser33Tyr (c.98C>A) (PubMed=9294210; PubMed=24755471; ATCC).

Homozygous for TGFBR2 p.Lys128Serfs*35 (c.383delA) (PubMed=12615714).

Has no TP53 mutation (PubMed=16418264).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        9,10

D3S1358        13,14,15

D5S818        10,14

D7S820        9,10

D8S1179        13,14,15

D13S317        11,12

D16S539        11,13

D18S51        13

D21S11        28,31

FGA        20

Penta D        10

Penta E        12,13

TH01        6,9.3

TPOX        8

vWA        18,20,21 (AddexBio; ATCC)

18,20 (Cosmic-CLP; ECACC; PubMed=25877200; PubMed=25926053)

参考文献

PubMed=29101300; DOI=10.15252/msb.20177701

Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.

Pharmacoproteomic characterisation of human colon and rectal cancer.

Mol. Syst. Biol. 13:951-951(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)