人肾癌细胞A498

细胞名称

人肾癌细胞A498                

货物编码

BFN60700271

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研用途               1类

培养体系

DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone）

培养温度

37℃

二氧化碳浓度

5%

简介

人肾癌细胞A498细胞系由AaronsonS建立，源自一位52岁患有肾癌的女性病人。贴壁培养，不规则形态。该细胞引种自ATCC HTB-44

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Part of: NCI-60 cancer cell line panel.

Doubling time: 58.4 hours (PubMed=22628656); 66.8 hours (NCI-DTP); ~62 hours (CLS); ~60 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Fluorescence phenotype profiling.

Omics: GPI-anchored proteins analysis by proteomics.

Omics: lncRNA expression profiling.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

Amelogenin        X

CSF1PO        11,12

D2S1338        26

D3S1358        15

D5S818        11,13

D7S820        11,12 (CLS)

10,11 (ATCC; CCRID; Cosmic-CLP; DSMZ; KCLB; PubMed=19372543; PubMed=25877200)

D8S1179        13,15

D13S317        12

D16S539        12

D18S51        17

D19S433        12

D21S11        28,32

FGA        18,20

Penta D        9,14

Penta E        10,14

TH01        6,9.3

TPOX        8,11 (ATCC; CCRID; CLS; Cosmic-CLP; DSMZ; KCLB; PubMed=19372543)

8 (PubMed=25877200)

vWA        18 (ATCC; CCRID; CLS; Cosmic-CLP; KCLB; PubMed=19372543; PubMed=25877200)

16,18 (DSMZ)

参考文献

PubMed=28489074; DOI=10.1038/ncomms15165

Sinha R., Winer A.G., Chevinsky M., Jakubowski C., Chen Y.-B., Dong Y., Tickoo S.K., Reuter V.E., Russo P., Coleman J.A., Sander C., Hsieh J.J., Hakimi A.A.

Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection.

Nat. Commun. 8:15165-15165(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)